Simulation and analysis on condensing effect of the different air suction slots of compact spinning with lattice apron. Journal of Donghua University (English Edition), 85(2), 160-163. The volume dataset is in the 07:00–19:00 period on December 15, 2016 (i.e., including 144 time slots), and the time span of the speed data source is December 10, 2018, to December 15, 2018, which are applied for further numerical tests.

With Selection-Based Processing, you can apply plug-in processing directly to one or more audio regions. Logic Pro creates a new file of the processed regions, and leaves the original files available in case you want to revert back to the originals. You can use any of the plug-ins included with Logic Pro, as well as Audio Units plug-ins from developers other than Apple. Selection-Based Processing is separate from any plug-ins that might exist on a track's channel strips.

To use Selection-Based Processing:

Select audio regions

You can select and process a part of a region, a whole region, or regions across multiple tracks.

1. Select one or more audio regions in the Tracks area or the Audio Track Editor, or use the Marquee tool to select parts of one or more regions in the Tracks area.
2. Choose Functions > Selection-Based Processing from the Tracks area or the Audio Track Editor local menu bar. The Selection-Based Processing window opens.

Choose channel strip settings or plug-ins

In the Selection-Based Processing window, you can set up two separate plug-ins chains, labeled 'A' and 'B' in the window. You can then toggle between the two plug-in chains and preview how the region will sound after processing. You can insert up to 15 plug-ins in each chain. You can choose complete channel strip settings, or you can insert individual plug-ins.

To choose a channel strip setting:

1. Under 'Plug-ins,' select the chain in which you want to insert a setting or a plug-in so it is highlighted blue.
2. Click the Channel Strip Setting pop-up menu and choose a setting from one of the submenus (Dynamics, Effects, Utility, or Vocal Tools).

To insert an individual plug-in:

• Click a slot in either plug-in chain and choose a plug-in from the menu.

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You can work with Selection-Based Processing channel strip settings the same way you work with standard channel strip settings.

Adjust the gain of the processed region

To adjust the gain of the region after it's processed, click the Gain pop-up menu and choose one of the following:

• No Change: The volume of the original source and the settings of the plug-ins determine the gain of the resulting audio file.
• Loudness Compensation: Logic Pro adjusts the volume of the processed file to match the original source recording.
• Overload Protection: If necessary, Logic Pro reduces volume just enough to prevent digital clipping. If peaks don't exceed the clipping threshold, then Logic Pro doesn't change the gain.
• Normalize: The volume of the processed file is adjusted so that the peaks fall just below the digital clipping threshold. This option would typically only be used on an entire region.

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Preview the processing

You can preview how your plug-ins or channel strip settings will affect your audio before applying the processing. Select the plug-in chain you want to preview, then click the Prelisten icon .

Click the Gear icon to set options for Prelisten:

• Prelisten enables Solo: Solos the region when prelistening.
• Prelisten enables Cycle: Loops the selected region or marquee selection.

You can bypass an entire plug-in chain by clicking the Bypass button at the top of the plug-in chain. You can bypass an individual plug-in by clicking its Bypass button in the slot.

Set how Logic Pro handles the processed audio regions

You can set how Logic Pro places audio regions in the Tracks area after you've applied processing:

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• Split at Marquee Borders: This option is available if you've selected part of a region for processing. When you select this option, Logic Pro creates a new audio file that encompasses only the length of the marquee selection.
• Create New Take: This option is available if you've accessed Selection-Based Processing from the Tracks area Function menu. When you select this option, Logic Pro creates a take folder that contains both the processed and unprocessed regions.
• Add Effect Tail:This option is available if you've selected a part of a region for processing.When you select this option,Logic Pro creates a region that includes any effect tail that may extend beyond the selection. However, the right border of the region is set at the marquee selection location. If the marquee selection is beyond the rightmost border of the region, the tail continues to its natural length. You can adjust the border to reveal the effect tail in the region.

Apply processing

After you've set all your options, select the plug-in chain that you want to use, then click Apply. You can undo any processing by clicking Undo.

You can also quickly apply the same processing to other selected regions. Select one or more regions, choose Functions > Selection-Based Processing from the Tracks area or Audio Track Editor local menu bar, then select 'Apply Selection-Based Processing Again.'

Overview

Test blots, as their name implies, are very simple Western blots that are created for the express purpose of optimizing or troubleshooting experimental conditions. They are usually produced by running multiple lanes of the same lysate or purified protein solution on a gel, and after transfer cutting the blot into strips to be tested individually.

They provide a quick and efficient means of examining a range of antibody dilutions or detection substrates

Dot blots and slot blots are also a very useful variation on the typical Western blot. They do not require gel electrophoresis, so there is no separation of proteins by size.

Instead, the target protein or cell lysate mixture is added directly onto the surface of the nitrocellulose or PVDF membrane. Protein solutions can be applied directly in a small volume, or with a vacuum manifold to produce an orderly grid of samples similar to that seen in Figure 14. Each dot or slot blot would contain known amounts of target protein or cell lysate.

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Once dry, dot blots and slot blots are subjected to the same immunodetection steps used for Western blotting, i.e. blocking, antibody incubation, and target detection with substrate. Grey and black spots on the figure below indicate which samples are positive for the target protein and correspond roughly to the bands produced on a Western blot.

Figure 14: Dot Blots and Slot Blots. The dot blots at the left represent a dilution series of a sample, with smaller lighter dots corresponding to lower concentrations of target protein. The slot blots represent a group of random samples, the intensity of the signal corresponds to the concentration of the target protein in that sample.

By making a number of identical dots or slots of a single known protein sample, or a range of sample dilutions, one can quickly test several combinations and concentrations of primary and secondary antibodies. Dot blots and slot blots are also beneficial when screening a large number of samples, or if a simple answer will suffice.

The main downside to slot blots/dot blots is that they provide no information about molecular weight. Thus, it is harder to detect false positive signals, or to tell whether modified forms of a protein are present.

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Detection with Substrate

Chapter 4: Data Analysis